Standard Operating Procedures

Documents

Several pathologists having already some experience in the field of Quality Management are presently joining their efforts to produce Standard Operating Procedures. These SOPs can be used as templates by European laboratories and can help them when writing down their own documentation for Quality Management. These documents are available below in PDF files.

General

  • Sample processing in the context of mollusc mortality events (1st edition, 2020)

 application/pdf SampleProcessing_1

Histo-cytopathology

  • Molluscs processing for diagnosis by histology (3rd edition, 2011)

 application/pdf Histology Process 3

  • Molluscs processing for diagnosis by cytology (1st edition, 2021)

application/pdf Cytology process 1

  • Diagnosis by histo-cytopathology of Bonamia spp. in flat oysters Ostrea spp. (5th edition, 2013)

application/pdf Bonamia sp. v5

  • Diagnosis by histo-cytopathology of Marteilia spp.  in the flat oyster Ostrea edulis and the mussels Mytilus edulis and M. galloprovincialis (2nd edition, 2009)

 

application/pdf Marteilia sp. 2

 

  • Diagnosis by histopathology of Perkinsus sp. in molluscs (1st edition, 2009)

 

application/pdf Perkinsus sp. 1

 

  • Diagnosis by histo-cytopathology of Mikrocytos sp. in oysters (2nd edition, 2010)

 

application/pdf Mikrocytos sp. 2

 

  • Diagnosis by histopathology of Haplosporidium sp. in oysters (1st edition, 2009)

 

application/pdf Haplosporidium sp. 1

 

  • Koehler illumination system for the microscope (1st edition, 2009)

 

application/pdf Microscope setting

 

Molecular biology

  • Bonamia spp detection by Polymerase Chain Reaction and species characterisation by Restriction Fragment Length Polymorphism (PCR-RFLP) (2nd edition, May 2019)

 

application/pdf Bonamia PCR-RFLP 2

  • Bonamia sp.detection and characterization by in situ hybridization (ISH) (1st edition, May 2013)

application/pdf Bonamia HIS v1

  • Bonamia ostreae and Bonamia exitiosa detection by Real time Polymerase Chain Reaction (PCR) (2nd edition, February 2023)

application/pdf B.ostreae and B.exitiosa RT PCR

  • Marteilia refringens detection and characterisation by Polymerase Chain Reaction- Restriction Fragment Length Polymorphism (PCR-RFLP) (3rd edition, December 2011)

application/pdf Marteilia PCR-RFLP_3

 

  •  Marteilia refringens detection and typing by Real time Polymerase Chain Reaction (PCR) (3a edition, March 2023)

application/pdf M.refringens type MO RealTime PCR 3a

  • Marteilia refringens and Bonamia sp. detection by Real Time Polymerase Chain Reaction (PCR) (1st edition, April 2017)

application/pdf Real time PCR Marteilia refringens and Bonamia sp. 1

  • Marteilia refringens detection by in situ hybridization (ISH) ( 4th edition,  August 2021)

application/pdf M.refringens_ISH_4

  • Perkinsus marinus detection by Real Time Polymerase Chain Reaction (PCR) ( 1st edition, April 2021)

application/pdf Pmarinus_RealTimePCR_1

  • Perkinsus marinus detection by in situ hybridization (ISH) (1st edition, August 2021)

application/pdf P.marinus_ISH_1

  • Mikrocytos mackini detection by conventional Polymerase Chain Reaction (PCR) (1st edition,  April 2021)

application/pdf Mmackini_PCR_1

  • Mikrocytos mackini detection by Real Time Polymerase Chain Reaction (PCR) (1st edition,  April 2021)

application/pdf Mmackini_RealTimePCR_1

  • Mikrocytos mackini detection by in situ hybridization (ISH) (1st edition,  August 2021)

application/pdf M.mackini_ISH_1

application/pdf OsHV-1 RTPCR_1

  • Vibrio aestuarianus detection by Real Time Polymerase Chain Reaction (PCR) (4th edition, March 2021)

application/pdf V.aestuarianus detection by Real-time PCR

  • Vibrio splendidus and V. aestuarianus detection by Real Time Polymerase Chain Reaction (PCR) (1st edition, October 2013)

application/pdf Vibrio splendidus and V. aestuarianus RT PCR

Other techniques

  • Quantification of Perkinsus sp. infection intensity using Ray’s Fluid Thioglycolate Medium (RFTM) Method (1st edition, February 2011)

application/pdf Perkinsus RFTM 1

  • Isolation of predominant bacteria in marine molluscs (1st edition, July 2015)

application/pdf Isolation of predominant bacteria in marine molluscs

If you cannot open the Pdf files, click here to get Adobe Reader

Quality management and Accreditation

Diagnosis of mollusc diseases by histology or by using molecular biology tools (PCR, HIS) is performed under a quality assurance system since 2003.

The EURL for mollusc diseases is accredited by the Cofrac (Comité français d'accréditation):

- for the diagnosis by histo-cytopathology of Bonamia sp., Marteilia sp., Perkinsus sp. and Mikrocytos sp. in molluscs (bivalves and gastropods) since October 2009 (ISO 17025 standard),

- for the organisation of Inter-Laboratory comparison test in histo-cytopathology since June 2021 (ISO 17043 standard)

- for the diagnosis of Bonamia ostreae, Bonamia exitiosa and Marteilia reringens by real-time PCR since March 2023 (ISO 17025 standard)

The laboratory is currently working to include real-time PCR assays for the diagnosis of exotic regulated pathogens Mikrocytos mackini and Perkinsus marinus in its scope of accreditation.

Cofrac testing Accreditation n° 1-2160,

Scope available on www.cofrac.fr

IFREMER ASIM- Unité Adaptation et Santé des Invertébrés Marins